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The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

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Overexpression of the gene has become observed in many tumors, which leads to chromosome instability, and therefore could contribute to tumorigenesis. This protein was uncovered to affiliate with, and be involved with, the phosphorylation of NPAT protein (nuclear protein mapped for the ATM locus), which participates in cell-cycle controlled histone gene expression and plays a critical purpose in selling cell-cycle development inside the absence of pRB. Two alternatively spliced transcript variants of this gene, which encode unique isoforms, happen to be explained. Two added splice variants were described but thorough nucleotide sequence info is not really but available.

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Blocking peptides are peptides that bind specifically for the concentrate on antibody and block antibody binding. These peptide generally is made up of the epitope regarded from the antibody.

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This tutorial outlines even more achievable optimization of cellular and immunoassay parameters to be certain the very get more info best final results are...

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This guideline outlines further possible optimization of cellular and immunoassay parameters to make sure the best possible outcomes are...

Antibodies bound to the blocking peptide not bind into the epitope about the concentrate on protein. By comparing the staining through the blocked antibody vs . the antibody alone, you can see which staining is unique.

Focus on Facts Cyclin E belongs on the hugely conserved cyclin loved ones, whose associates are characterised by a extraordinary periodicity in protein abundance from the cell cycle. Cyclins functionality as regulators of CDK kinases. Distinct cyclins exhibit distinctive expression and degradation designs which contribute into the temporal coordination of every mitotic occasion. Cyclin E forms a posh with and functions to be a regulatory subunit of CDK2, whose action is needed for cell cycle G1/S transition. This protein accumulates at the G1-S period boundary and it is degraded as cells development by way of S phase.

The measurement of protein phosphorylation is a useful tool for measuring the modulation of receptor activation by both of those antibodies...

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